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    Validating the genomic signature of pediatric septic shock

    To this end, it integrates expression data of different types (ESTs, hybridization, Affymetrix and RNA-Seq data, as of Bgee release 12), from different species (fruit fly, human, mouse, Xenopus and zebrafish, as of Bgee release 12).Each data type is analysed using dedicated statistical tests, to generate present/absent expression calls, and differential expression calls (overexpression and underexpression).We report that analytical variance caused by sample processing was acceptably small.

    validating the genomic signature of pediatric septic shock-87validating the genomic signature of pediatric septic shock-34

    Database URL: Bgee (a data Base for Gene Expression Evolution) (1) is a resource to perform high-throughput and automated comparisons of gene expression patterns between species.We analyzed sources of variance in genome-wide expression analyses performed with commercial oligonucleotide arrays.In addition, variance in gene expression in human blood leukocytes caused by repeated sampling in the same subject, among different healthy subjects, among different leukocyte subpopulations, and the effect of traumatic injury, were also explored.In the United States, sepsis is one of the top ten leading causes of mortality [4].Although adjusted in-hospital mortality has decreased gradually (2-3% per year) according to a recent report [1, 5], sepsis associated mortality remained high, from 50/100,000 to 75/100,000 [1, 6].

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